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On E9th Pedal Steel>
Our open tuning is an E tuning with no pedals and a "A" tuning with pedals down.
We are so used to tuning to E 440 but the rest of the world is tuned to A 440.
So if you tune your guitar to E 440 and play an open A chord with pedals down; than you are out of tune with the rest of the world because you "A" note(chord) will be flat (Mainly because of the pull on the guitar when pushing pedals; will drop notes).
So since I and the rest of us are used to tuning to the E note on a Pedal steel guitar from the start; what I would suggest is to push "a" and "b" pedals down and while down; pick your "E" note(highest open "E") and tune it to 440 on your tuner. Now let off your pedals and play that "E" note again and look at where it now lays on your tuner(usually sharp to 440); and that is your "NEW" E note and go ahead and retune the rest of your guitar to that E note reference like your used to(and now with pedals down; your "A" note will be in tune to E440 because your pedals down "E" note is 440.
So the way it turns out for your particular guitar is how much difference there is in the way your guitar re-acts to the pedals pushed.
So now in all probability your open E reference is sharp to E 440; but your "A" chord is now tuned to "A" 440 and you will play in better tune than you ever have; if your not already doing this. It is ok to the ear to be slightly sharp.....but it is never ok to be flat. Sharp adds excitement; and Flat adds Death>to the music.
I alway tune my guitar by ear; as Tom Brumley once told me: "Ricky if you always practice on tuning your guitar by ear; than you are practicing on playing in tune and that is the goal here"!!!.
I do have the notes somewhat memorized where they lay on my tuner for my guitar, for the times that I don't get to make any noise at the gig or don't have time or can't hear. But finding out how your guitar tunes to "A" 440 is VERY important.
Another little practice thing I do at home when I ever practice; is I never practice with reverb and after tuning my steel; I will turn on my metronome that has a "A" 440 pitch to it; and I just warm up and play along with that "A" pitch going and you can play in key of A or E or D or C and I really listen to playing in tune with that pitch while I warm up or work on what ever.

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First you tune your "A" note to 440 then with
pedals down tune your "E" note to that "A" 440
note. Now you have the new "E" note reference to
"A" 440.
Tuning the open tuning.
Tune the other E note(4and8)
Tune the G#'s (3and6)to E
Tune the B's (5and10)to E
Tune the F#'s (1and7)to B
Tune the D# to B(2nd string)
Tuning the pedals and knee levers
Tune the A pedal(5and10) to the E note
Tune the B pedal(3and6)to the E note("A"440)
Tune the C pedal(4and5)to the A note
Tune E lower knee(4and8)to the B note
Tune E raise knee(4and8) to the A pedal
Tune D note(9th string) to and "A" note
Tune D# lower 1/2 tone to 9th string
Tune D# lower whole tone to A pedal

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